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- W99673759 abstract "Summary Polysaccharides are traditionally modified post-extraction either chemically or by the application of enzymes. The ability to modify plants genetically has now provided an alternative, in vivo, approach to the production of designer polymers. There are perhaps two basic ways in which plants may be modified to alter the structure of their polysaccharides. The first would be the addition of novel enzymes to alter the biosynthetic or degradative pathways. The second would again achieve alterations to these pathways but would involve the silencing of genes for specific enzymes. The genetic tools for the manipulation of the biosynthetic pathways are limited at present. However, those for the degradative pathways are more readily obtainable. Genes may be silenced by the use of either antisense or co-suppression technology. Tomato h i t represent a good model system for testing this approach to polysaccharide modification. The tomato plant is readily transformable and the h i t cell wall pectin undergoes extensive modification during ripening. Tomato pectin has been modified in vivo by the silencing of genes for three pecteolytic enzymes namelypolygalacturonase, pectinesterase and galactosidase. These genetic modifications result in alterations to both pectin structure and its functionality in tomato products. The effects on pectin structure are fairly predictable, the effects on functionality less so in that it would appear that there is essential enzyme interaction responsible for the viscosity of tomato pastes." @default.
- W99673759 created "2016-06-24" @default.
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- W99673759 date "2000-01-01" @default.
- W99673759 modified "2023-09-26" @default.
- W99673759 title "GENETIC ENGINEERING AS A MEANS TO MODIFY POLYSACCHARIDES" @default.
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- W99673759 doi "https://doi.org/10.1533/9781845698355.6.397" @default.
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