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- W99683336 abstract "Publisher Summary This chapter describes the maintenance of diploid fibroblast cultures as nonmiotic populations. The use of normal diploid fibroblasts for in vitro aging studies has become well established. The majority of these investigations have employed actively proliferating populations; however, in situ fibroblasts of connective tissue have little or no mitotic activity except during wound healing. A closer approximation to the in vivo situation might be the use of nonmitotic cell populations for aging studies in vitro. Confluent cultures, derived from stock lines carried in antibiotic-free medium, are subcultured at a 1:4 split ratio and grown to confluency in McCoy's Medium 7a supplemented with 10% fetal bovine or whole calf serum and 25 μg/ ml of both penicillin and streptomycin. The incubation medium is changed every 48 hours until confluency is reached. At this time, the growth medium is decanted and replaced with McCoy's Medium 7a supplemented with 0.5% serum and antibiotics. The cultures are incubated in a closed system under an atmosphere of 8% CO2. The incubation medium is changed twice a week for the duration of the maintenance period." @default.
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- W99683336 date "1973-01-01" @default.
- W99683336 modified "2023-10-16" @default.
- W99683336 title "Maintenance of Diploid Fibroblast Cultures as Nonmitotic Populations" @default.
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- W99683336 doi "https://doi.org/10.1016/b978-0-12-427150-0.50063-2" @default.
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