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- W99693212 abstract "We report here an improved method for analyzing protein surface expression utilizing a cold-adapted trypsin. Preservation of activity of the enzyme at 0–4°C permits modification of the protease method of surface analysis to temperatures at which trafficking of mammalian plasmalemmal proteins is blocked. This is an important advantage over established trypsin-cleavage protocols. Moreover, the method is less time-consuming than surface biotinylation." @default.
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- W99693212 date "2011-04-01" @default.
- W99693212 modified "2023-10-13" @default.
- W99693212 title "Cold-adapted protease enables quantitation of surface proteins in the absence of membrane trafficking" @default.
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- W99693212 doi "https://doi.org/10.2144/000113651" @default.
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