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- W997149512 abstract "Several enzymatic activities have been described in the purified virions of RNA tumor viruses. This chapter describes two activities that have been extensively purified: DNA polymerase that can utilize both ribo- and deoxyribonucleotide templates and ribonuclease H that selectively degrades RNA from an RNA-DNA hybrid. Several laboratories have purified DNA polymerase from avian myeloblastosis virus (AMV), Rous sarcoma virus, and Rauscher murine leukemia virus, by using a combination of ion-exchange chromatography, gel filtration, and sedimentation velocity centrifugation. The chapter describes the method of purification of AMV DNA polymerase. The enzyme is approximately 100-fold purified. Its response to various templates is discussed. The chapter concludes with a discussion on the transcription of various templates by purified AMV DNA polymerase. Purified DNA polymerase can utilize a very wide variety of polyribo and deoxyribonucleotides as templates. A primer is required to initiate DNA synthesis." @default.
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- W997149512 date "1974-01-01" @default.
- W997149512 modified "2023-10-14" @default.
- W997149512 title "[13] Purification of the RNA-directed DNA polymerase from avian myeloblastosis virus and its assay with polynucleotide templates" @default.
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- W997149512 doi "https://doi.org/10.1016/0076-6879(74)29015-3" @default.
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