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- W998057954 abstract "Publisher Summary This chapter describes the latter category of Transposase (Tn5) mutagenesis experiments. Application to the study of the structure of cloned genes with a readily identifiable phenotype in E. coli (or related bacteria) and of genetic organization in Agrobacterium and (fast growing) rhizobia is emphasized. Two protocols for Tn5 mutagenesis of cloned deoxyribo nucleic acid (DNA) segments in E. coli, and two protocols for gene replacement with Tn5-mutated segments will be discussed. Parameters of transposon Tn5 that are pertinent to the mutagenesis protocols are described in this chapter: vectors used for transposition frequency, insertional specificity, and polarity and stability of Tn5 insertions have been discussed in detail. One parameter is of particular interest when trying to generate a population of “random” Tn5 insertions in a cloned DNA segment— that is, insertional specificity or the absence of “hot spots” of Tn5 insertion. The utility of Tn5 has recently been extended by the construction of Tn5 derivatives carrying other selectable marker genes." @default.
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- W998057954 date "1987-01-01" @default.
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- W998057954 title "[11] Transposon Tn5 mutagenesis to map genes" @default.
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- W998057954 doi "https://doi.org/10.1016/0076-6879(87)54077-0" @default.
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