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- W998543078 abstract "Publisher Summary The chapter describes methods for the production of human minisegregant cells from mitotic precursors. The aim is to assess the nature of the perturbation induced in the mitotic cell, and the roles of substances that either inhibit or promote this aberrant division. The chapter also describes the fractionation of minisegregant cells and some of their properties and helps to assess their competence to transfer information by means of cell fusion. The ability to fragment human cells by avariety of simple procedures and to produce DNA-containing minisegregants makes possible new experiments in somatic cell genetics. Reconstitution experiments between whole cells and cell fragments will form a useful contrast and complement to whole-cell fusions and should help to simplify analysis of regulatory phenomena, not least by predetermining the direction of chromosome balance in the hybrid. As minisegregants and microcells can be separated according to size and DNA content, it should be possible to control the amount of DNA introduced by these agents and therefore to construct the desired hybrid immediately." @default.
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- W998543078 date "1978-01-01" @default.
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- W998543078 title "Chapter 19 Human Minisegregant Cells" @default.
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- W998543078 doi "https://doi.org/10.1016/s0091-679x(08)62023-3" @default.
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