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- W999621277 abstract "目的：研究碱性成纤维细胞生长因子（basic fibroblast growth factor，bFGF）、成纤维细胞生长因子受体1（fibroblast growth factor receptor 1，FGFR1）及bFGF mRNA三者与肿瘤细胞（神经胶质瘤细胞）恶性行为的相关性。方法：设计、合成bFGF反义寡核苷酸，转染SWO-38神经胶质瘤细胞，用原位杂交、免疫组化及图像分析、间接ELISA、细胞增殖MTT法、细胞集落形成等方法，分别检测转染前后bFGF、FGFR1、bFGF mRNA表达的改变，观察不同剂量bFGF反义寡核苷酸转染后细胞生长、集落形成的变化。结果：大部分细胞bFGF mRNA和细胞生长可被bFGF反义寡核苷酸抑制，细胞生长的抑制呈浓度依赖性，最高抑制率可达到48％，其抑制作用可被外源性bFGF完全逆转；反义寡核苷酸处理组大部分细胞表达的FGFR1减少、细胞分泌的bFGF明显减少，集落形成抑制率达35％．外源性bFGF的逆转作用为8％。正义组和阴性对照组均无以上的明显改变。结论：揭示了反义寡核苷酸可特异性抑制bFGF mRNA和肿瘤细胞的bFGF合成表达，但不能拮抗外源性bFGF结合bFGFR的生物学作用；FGFR1随肿瘤细胞SWO-38中bFGF表达水平的降低而下调。" @default.
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- W999621277 date "2006-01-01" @default.
- W999621277 modified "2023-09-23" @default.
- W999621277 title "bFGF mRNA、bFGF及FGFR1与神经胶质瘤细胞恶性行为的相关性" @default.
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