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- W999827593 abstract "Publisher Summary This chapter describes the application of data gathered from various sources that are combined to refine the radioreceptor assays for two putative neurotransmitters in the central nervous system, namely, neurotensin and encephalin. Neurotensin is translated from a single mRNA sequence containing a related peptide, neuromedin N. The addition of a radiactive isotope to biologically active peptides has greatly aided understanding of how the peptides interact with their effector systems. The choice of tracer or radioisotope is critical in defining the limits of the assay system and the results that can be anticipated. The most preferable methodology for the separation and purification of radioiodinated molecules is high-performance liquid chromatography (HPLC). Reversed-phase HPLC or ion-exchange matrices can be employed for the separation of neuropeptides. After drying, tissue sections are apposed to autoradiographic film or an emulsion in a dry environment to produce the autoradiographic image." @default.
- W999827593 created "2016-06-24" @default.
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- W999827593 date "1991-01-01" @default.
- W999827593 modified "2023-09-27" @default.
- W999827593 title "Radioiodinated Ligands in Assays for Neurotensin and Enkephalin Receptors" @default.
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- W999827593 doi "https://doi.org/10.1016/b978-0-12-185259-7.50036-3" @default.
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