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- PCL_0016451 IAO_0000028 "Human M1 snSMART-Seq" @default.
- PCL_0016451 PCL_0010061 "10534" @default.
- PCL_0016451 archivedAt "https://assets.nemoarchive.org/dat-ci7q9k4" @default.
- PCL_0016451 assesses "human" @default.
- PCL_0016451 discussionUrl "https://celltypes.brain-map.org/rnaseq/human_m1_10x?selectedVisualization=Heatmap&colorByFeature=Cell+Type&colorByFeatureValue=GAD1" @default.
- PCL_0016451 includedInDataCatalog CCN201912131 @default.
- PCL_0016451 position "M1" @default.
- PCL_0016451 type Dataset @default.
- PCL_0016451 type NamedIndividual @default.
- PCL_0016451 comment "A single cell sequencing assay wherein the target cells are lysed, and the RNA is hybridized to an oligo(dT)-containing primer. The first strand of the cDNA is synthesized with the addition of a few untemplated C nucleotides. This poly(C) overhang is added exclusively to full-length transcripts. An oligonucleotide primer is hybridized to the poly(C) overhang and used to synthesize the second strand. Full-length cDNAs are PCR-amplified to obtain nanogram amounts of DNA. The PCR products are purified for sequencing. This is the fourth version of the assay." @default.
- PCL_0016451 isDefinedBy pcl.owl @default.
- PCL_0016451 label "Human M1 (CCN201912131) single nuclei SMART-Seq v4" @default.
- PCL_0016451 prefLabel "SMART-Seq v4" @default.