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- PCL_0016454 IAO_0000028 "Human M1 SNARE-seq2" @default.
- PCL_0016454 PCL_0010061 "84178" @default.
- PCL_0016454 archivedAt "https://knowledge.brain-map.org/data/TGNO3HM2KO03B5EWGBT/summary" @default.
- PCL_0016454 assesses "human" @default.
- PCL_0016454 discussionUrl "https://nemoanalytics.org//index.html?layout_id=ac9863bf" @default.
- PCL_0016454 includedInDataCatalog CCN201912131 @default.
- PCL_0016454 position "M1" @default.
- PCL_0016454 type Dataset @default.
- PCL_0016454 type NamedIndividual @default.
- PCL_0016454 comment "A sequencing assay that involves the isolation of single nuclei and open chromatin tagmentation in the isolated nuclei using a transposase. The tagmented nuclei are then encapsulated in a droplet including both an oligo-dT-containing barcoded bead and a splint oligonucleotide, which links the tagmented gDNA fragments to the bead, enabling the bead to capture both mRNAs and open chromatin fragments. After mRNAs and gDNA fragments are released from the bead by heating, RT and PCR amplification are performed to generate a library of cDNA and open chromatin gDNA fragments. " @default.
- PCL_0016454 comment "A sequencing assay that involves the isolation of single nuclei and open chromatin tagmentation in the isolated nuclei using a transposase. The tagmented nuclei are then encapsulated in a droplet including both an oligo-dT-containing barcoded bead and a splint oligonucleotide, which links the tagmented gDNA fragments to the bead, enabling the bead to capture both mRNAs and open chromatin fragments. After mRNAs and gDNA fragments are released from the bead by heating, RT and PCR amplification are performed to generate a library of cDNA and open chromatin gDNA fragments." @default.
- PCL_0016454 isDefinedBy pcl.owl @default.
- PCL_0016454 label "Human M1 (CCN201912131) single nuclei SNARE-seq2" @default.
- PCL_0016454 prefLabel "SNARE-seq2" @default.