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- PCL_0020453 IAO_0000028 "Marmoset M1 snmC-Seq2" @default.
- PCL_0020453 PCL_0010061 "9946" @default.
- PCL_0020453 archivedAt "https://knowledge.brain-map.org/data/TGNO3HM2KO03B5EWGBT/summary" @default.
- PCL_0020453 assesses "marmoset" @default.
- PCL_0020453 discussionUrl "https://nemoanalytics.org//index.html?layout_id=ac9863bf" @default.
- PCL_0020453 headline "CCN201912132" @default.
- PCL_0020453 includedInDataCatalog CCN201912132 @default.
- PCL_0020453 position "M1" @default.
- PCL_0020453 type Dataset @default.
- PCL_0020453 type NamedIndividual @default.
- PCL_0020453 comment "A sequencing assay that involves the isolation of single nuclei and open chromatin tagmentation in the isolated nuclei using a transposase. The tagmented nuclei are then encapsulated in a droplet including both an oligo-dT-containing barcoded bead and a splint oligonucleotide, which links the tagmented gDNA fragments to the bead, enabling the bead to capture both mRNAs and open chromatin fragments. After mRNAs and gDNA fragments are released from the bead by heating, RT and PCR amplification are performed to generate a library of cDNA and open chromatin gDNA fragments. " @default.
- PCL_0020453 comment "A sequencing assay that involves the isolation of single nuclei and open chromatin tagmentation in the isolated nuclei using a transposase. The tagmented nuclei are then encapsulated in a droplet including both an oligo-dT-containing barcoded bead and a splint oligonucleotide, which links the tagmented gDNA fragments to the bead, enabling the bead to capture both mRNAs and open chromatin fragments. After mRNAs and gDNA fragments are released from the bead by heating, RT and PCR amplification are performed to generate a library of cDNA and open chromatin gDNA fragments." @default.
- PCL_0020453 isDefinedBy pcl.owl @default.
- PCL_0020453 label "Marmoset M1 (CCN201912132) single nuclei snmC-Seq2" @default.
- PCL_0020453 prefLabel "SNARE-seq2" @default.